Inw ard current generated by N a-C a exchange during the action potential in single atrial cells of the rabb it

To investigate the underlying ionic mechanism of the late plateau phase of the action potential in rabbit atrium the whole-cell patch-clamp tech­ nique with intracellular perfusion was used. We recorded the inward current during repolarizations following a brief 2 ms depolarizing pulse to +40 mV from a holding potential of between —70 and —80 mV. The development of this current coincides with the onset of the late plateau phase of the action potential. Peak activation of the current occurs about 10 ms from the beginning of the depolarizing pulse, and it decays spon­ taneously with a slow timecourse. Its voltage dependency from —40 mV to +40 mV shows very steep activation ( — 40 to —20 mV) and shows almost the same maximum magnitude between —10 mV and +40 mV. This behaviour is quite different from that of the calcium current. The inward current and the late plateau phase of the action potential were both abolished by the application of 5 raM EGTA, 1 gM ryanodine and by reducing the Na+ gradient. The fully activated current-voltage relation of the inward current was plotted as the difference current before and after treatment with Ryanodine, Diltiazem, 20 mM Na+ inside or 30 % Na+ outside and shows an exponential voltage dependence with the largest magnitude of the current occurring at negative potentials. The currentvoltage (I-V) curve was well fitted by the Na-Ca exchange equation, i = A exp (— (1 — r) EF / RT) .The results suggest that the inward current contributes to the generation of the late plateau phase of the rabbit atrial action potential, and is activated by intracellular calcium released from the sarcoplasmic reticulum. Sarcoplasmic reticulum calcium release appears to be triggered both by the membrane voltage and by the calcium current. It is concluded that the inward current is generated by Na-Ca exchange.